Identification of Plasmid-Encoded sRNAs in a bla_NDM-1-Harboring Multidrug-Resistance Plasmid pNDM-HK in Enterobacteriaceae.

ABSTRACT: Small RNAs (sRNAs) play significant roles in regulating gene expression post-transcriptionally in response to environmental changes in bacteria. In this work, we identified and characterized six novel sRNAs from an emerging multidrug-resistance (MDR) plasmid pNDM-HK, a New Delhi metallo-β-lactamase 1 gene (bla_NDM-1)-carrying IncL/M plasmid that has caused worldwide threat in recent years. These sRNAs are located at different regions of pNDM-HK, such as replication, stability, and variable regions. Moreover, one of the plasmid-encoded sRNAs (NDM-sR3) functions in an Hfq-dependent manner and possibly plays roles in the fitness of pNDM-HK carrying bacteria. In addition, we attempted to construct the phylogenetic tree based on these novel sRNAs and surprisingly, the sRNA-phyl

SUBMITTER: Kong HK 

PROVIDER: S-EPMC5880898 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature