Identifying RNA splicing factors using IFT genes in Chlamydomonas reinhardtii.

ABSTRACT: Intraflagellar transport moves proteins in and out of flagella/cilia and it is essential for the assembly of these organelles. Using whole-genome sequencing, we identified splice site mutations in two IFT genes, IFT81 (fla9) and IFT121 (ift121-2), which lead to flagellar assembly defects in the unicellular green alga Chlamydomonas reinhardtii The splicing defects in these ift mutants are partially corrected by mutations in two conserved spliceosome proteins, DGR14 and FRA10. We identified a dgr14 deletion mutant, which suppresses the 3' splice site mutation in IFT81, and a frameshift mutant of FRA10, which suppresses the 5' splice site mutation in IFT121 Surprisingly, we found dgr14-1 and fra10 mutations suppress both splice site mutations. We suggest these two proteins are involved in fac

SUBMITTER: Lin H 

PROVIDER: S-EPMC5881031 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature