Microbial rRNA Synthesis and Growth Compared through Quantitative Stable Isotope Probing with H₂¹⁸O.

ABSTRACT: Growing bacteria have a high concentration of ribosomes to ensure sufficient protein synthesis, which is necessary for genome replication and cellular division. To elucidate whether metabolic activity of soil microorganisms is coupled with growth, we investigated the relationship between rRNA and DNA synthesis in a soil bacterial community using quantitative stable isotope probing (qSIP) with H₂¹⁸O. Most soil bacterial taxa were metabolically active and grew, and there was no significant difference between the isotopic composition of DNA and RNA extracted from soil incubated with H₂¹⁸O. The positive correlation between ¹⁸O content of DNA and rRNA of taxa, with a slope statistically indistinguishable from 1 (slope = 0.96; 95% confidence

SUBMITTER: Papp K 

PROVIDER: S-EPMC5881069 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature