MiRNA extraction from cell-free biofluid using protein corona formed around carboxyl magnetic nanoparticles.

ABSTRACT: MicroRNA (miRNA) in urine has been considered as a potential biomarker for early-stage diagnosis of multiple diseases like urinary system cancer, kidney injury and diabetes, owing to their many demonstrated advantages including long-term stability and noninvasiveness. However, the traditional enrichment and extraction processes of miRNAs from urine are cumbersome and tedious due to the low concentration and multiple carriers of miRNAs. Herein, we present a novel method to collect low concentrations of miRNAs from dilute solutions such as urine and cell culture medium. 10-nm core sized magnetic nanoparticles with carboxylic acid coating can adsorb low-concentration proteins, and form protein corona which makes them easy to aggregate and precipitate for subsequent isolation. In urine and cel

SUBMITTER: Xu S 

PROVIDER: S-EPMC5881906 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature