All three quinone species play distinct roles in ensuring optimal growth under aerobic and fermentative conditions in E. coli K12.

ABSTRACT: The electron transport chain of E. coli contains three different quinone species, ubiquinone (UQ), menaquinone (MK) and demethylmenaquinone (DMK). The content and ratio of the different quinone species vary depending on the external conditions. To study the function of the different quinone species in more detail, strains with deletions preventing UQ synthesis, as well as MK and/or DMK synthesis were cultured under aerobic and anaerobic conditions. The strains were characterized with respect to growth and product synthesis. As quinones are also involved in the control of ArcB/A activity, we analyzed the phosphorylation state of the response regulator as well as the expression of selected genes.The data show reduced aerobic growth coupled to lactate production in the mutants defective in ub

SUBMITTER: Nitzschke A 

PROVIDER: S-EPMC5882134 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature