Promotion of malignant phenotype after disruption of the three-dimensional structure of cultured spheroids from colorectal cancer.

ABSTRACT: Individual and small clusters of cancer cells may detach from the edges of a main tumor and invade vessels, which can act as the origin of metastasis; however, the mechanism for this phenomenon is not well understood. Using cancer tissue-originated spheroids, we studied whether disturbing the 3D architecture of cancer spheroids can provoke the reformation process and progression of malignancy. We developed a mechanical disruption method to achieve homogenous disruption of the spheroids while maintaining cell-cell contact. After the disruption, 9 spheroid lines from 9 patient samples reformed within a few hours, and 3 of the 9 lines exhibited accelerated spheroid growth. Marker expression, spheroid forming capacity, and tumorigenesis indicated that stemness increased after spheroid disrupti

SUBMITTER: Piulats JM 

PROVIDER: S-EPMC5882311 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature