Quantitative Analysis of Immune Infiltrates in Primary Melanoma.

ABSTRACT: Novel methods to analyze the tumor microenvironment (TME) are urgently needed to stratify melanoma patients for adjuvant immunotherapy. Tumor-infiltrating lymphocyte (TIL) analysis, by conventional pathologic methods, is predictive but is insufficiently precise for clinical application. Quantitative multiplex immunofluorescence (qmIF) allows for evaluation of the TME using multiparameter phenotyping, tissue segmentation, and quantitative spatial analysis (qSA). Given that CD3⁺CD8⁺ cytotoxic lymphocytes (CTLs) promote antitumor immunity, whereas CD68⁺ macrophages impair immunity, we hypothesized that quantification and spatial analysis of macrophages and CTLs would correlate with clinical outcome. We applied qmIF to 104 primary stage II to III melanoma tumor

SUBMITTER: Gartrell RD 

PROVIDER: S-EPMC5882545 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature