On the design of CRISPR-based single-cell molecular screens.

ABSTRACT: Several groups recently coupled CRISPR perturbations and single-cell RNA-seq for pooled genetic screens. We demonstrate that vector designs of these studies are susceptible to ∼50% swapping of guide RNA-barcode associations because of lentiviral template switching. We optimized a published alternative, CROP-seq, in which the guide RNA also serves as the barcode, and here confirm that this strategy performs robustly and doubled the rate at which guides are assigned to cells to 94%.

SUBMITTER: Hill AJ 

PROVIDER: S-EPMC5882576 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature