Development of a Cell-Based Assay to Assess Binding of the proNGF Prodomain to Sortilin.

ABSTRACT: Sortilin was first identified based on its activity as part of intracellular protein sorting machinery. Recently, it was discovered that sortilin also acts as a cell surface receptor for the propeptide form of nerve growth factor (proNGF), progranulin, and neurotensin. The interaction of sortilin to these neurotrophic ligands is linked to diseases of the nervous system that lead to neurodegeneration and neuropathic pain. Blocking of the interaction of sortilin to these ligands may prevent or slow the progress of these nervous system disorders. In vitro screening assays for blocking compounds or peptides are part of the standard set of tools for drug discovery. However, assays for sortilin biology are not readily available to determine if the selected blocking agent inhibits sortilin activi

SUBMITTER: Malik I 

PROVIDER: S-EPMC5882628 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature