Non-invasive paper-based microfluidic device for ultra-low detection of urea through enzyme catalysis.

ABSTRACT: This work describes the design, fabrication and characterization of a paper-based microfluidic device for ultra-low detection of urea through enzyme catalysis. The microfluidic system comprises an entry port, a fluidic channel, a reaction zone and two electrodes (contacts). Wax printing was used to create fluidic channels on the surface of a chromatography paper. Pre-conceptualized designs of the fluidic channel are wax-printed on the paper substrate while the electrodes are screen-printed. The paper printed with wax is heated to cause the wax reflow along the thickness of the paper that selectively creates hydrophilic and hydrophobic zones inside the paper. Urease immobilized in the reaction zone catalyses urea into releasing ions and, thereby, generating a current flow between the electr

SUBMITTER: Suresh V 

PROVIDER: S-EPMC5882721 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature