Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing.

ABSTRACT: Recent studies have shown that the transcription factor Foxn1, which is expressed in keratinocytes, is involved in the skin wound healing process, yet how Foxn1 functions remains largely unknown. Our latest data indicate that Foxn1 drives skin healing via engagement in re-epithelization and the epithelial-mesenchymal transition (EMT) process. In the present study, 2D-DIGE proteomic profiling analysis of in vitro cultured keratinocytes transfected with adenoviral vector carrying Foxn1-GFP or GFP alone (control) revealed forty proteins with differential abundance between the compared groups. Among the proteins with Foxn1-dependent expression, several enable adaptation to hypoxia. Subsequent experiments revealed that hypoxic conditions (1% O₂) stimulate endogenous and exogenous (tr

SUBMITTER: Kur-Piotrowska A 

PROVIDER: S-EPMC5882803 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature