Physiological and transcriptome changes induced by Pseudomonas putida acquisition of an integrative and conjugative element.

ABSTRACT: Integrative and conjugative elements (ICEs) comprise ubiquitous large mobile regions in prokaryotic chromosomes that transmit vertically to daughter cells and transfer horizontally to distantly related lineages. Their evolutionary success originates in maximized combined ICE-host fitness trade-offs, but how the ICE impacts on the host metabolism and physiology is poorly understood. Here we investigate global changes in the host genetic network and physiology of Pseudomonas putida with or without an integrated ICEclc, a model ICE widely distributed in proteobacterial genomes. Genome-wide gene expression differences were analyzed by RNA-seq using exponentially growing or stationary phase-restimulated cultures on 3-chlorobenzoate, an aromatic compound metabolizable thanks to specific ICEclc-l

SUBMITTER: Miyazaki R 

PROVIDER: S-EPMC5882942 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature