Transient reduction of DNA methylation at the onset of meiosis in male mice.

ABSTRACT:

Background

Meiosis is a specialized germ cell cycle that generates haploid gametes. In the initial stage of meiosis, meiotic prophase I (MPI), homologous chromosomes pair and recombine. Extensive changes in chromatin in MPI raise an important question concerning the contribution of epigenetic mechanisms such as DNA methylation to meiosis. Interestingly, previous studies concluded that in male mice, genome-wide DNA methylation patters are set in place prior to meiosis and remain constant subsequently. However, no prior studies examined DNA methylation during MPI in a systematic manner necessitating its further investigation.

Results

In this study, we used genome-wide bisulfite sequencing to determine DNA methylation of adult mouse spermatocytes at all MPI substages, spermatog

SUBMITTER: Gaysinskaya V 

PROVIDER: S-EPMC5883305 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature