Lipid accumulation in human breast cancer cells injured by iron depletors.

ABSTRACT:

Background

Current insights into the effects of iron deficiency in tumour cells are not commensurate with the importance of iron in cell metabolism. Studies have predominantly focused on the effects of oxygen or glucose scarcity in tumour cells, while attributing insufficient emphasis to the inadequate supply of iron in hypoxic regions. Cellular responses to iron deficiency and hypoxia are interlinked and may strongly affect tumour metabolism.

Methods

We examined the morphological, proteomic, and metabolic effects induced by two iron chelators-deferoxamine (DFO) and di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT)-on MDA-MB-231 and MDA-MB-157 breast cancer cells.

Results

These chelators induced a cytoplasmic massive vacuolation and accumulation of lipid dr

SUBMITTER: De Bortoli M 

PROVIDER: S-EPMC5883539 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature