Enhanced Ordering in Monolayers Containing Glycosphingolipids: Impact of Carbohydrate Structure.

ABSTRACT: The influence of carbohydrate structure on the ordering of glycosphingolipids (GSLs) and surrounding phospholipids was investigated in monolayers at the air-water interface. Binary mixtures composed of GSLs, chosen to span a range of carbohydrate complexity, and zwitterionic dipalmitoylphosphatidylcholine phospholipid, were studied. X-ray reflectivity was used to measure the out-of-plane structure of the monolayers and characterize the extension and conformation of the GSL carbohydrates. Using synchrotron grazing incidence x-ray diffraction, the in-plane packing of the lipid acyl chains and the area per molecule within ordered domains were characterized at different mole ratios of the two components. Our findings indicate that GSL-containing mixtures, regardless of the carbohydrate size, e

SUBMITTER: Watkins EB 

PROVIDER: S-EPMC5883550 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature