Characterization of Cell Boundary and Confocal Effects Improves Quantitative FRAP Analysis.

ABSTRACT: Fluorescence recovery after photobleaching (FRAP) is an important tool used by cell biologists to study the diffusion and binding kinetics of vesicles, proteins, and other molecules in the cytoplasm, nucleus, or cell membrane. Although many FRAP models have been developed over the past decades, the influence of the complex boundaries of 3D cellular geometries on the recovery curves, in conjunction with regions of interest and optical effects (imaging, photobleaching, photoswitching, and scanning), has not been well studied. Here, we developed a 3D computational model of the FRAP process that incorporates particle diffusion, cell boundary effects, and the optical properties of the scanning confocal microscope, and validated this model using the tip-growing cells of Physcomitrella patens. We

SUBMITTER: Kingsley JL 

PROVIDER: S-EPMC5883553 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature