Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors.

ABSTRACT:

Background

The expression of a specific combination of transcription factors (TFs) in the multipotent progenitor cells (MPCs) is critical for determining pancreatic cell fate. NKX6.1 expression in PDX1⁺ MPCs is required for functional β cell generation. We have recently demonstrated the generation of a novel population of human pluripotent stem cell (hPSC)-derived MPCs that exclusively express NKX6.1, independently of PDX1 (PDX1^-/NKX6.1⁺). Therefore, the aim of this study was to characterize this novel population to elucidate its role in pancreatic development.

Methods

The hPSCs were exposed to two differentiation protocols to generate MPCs that were analyzed using different techniques.

Results

Based on the expression of PDX1 and NKX6.

SUBMITTER: Aigha II 

PROVIDER: S-EPMC5883581 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature