Cryo-EM Elucidation of the Structure of Bacteriophage P22 Virions after Genome Release.

ABSTRACT: Genome ejection proteins are required to facilitate transport of bacteriophage P22 double-stranded DNA safely through membranes of Salmonella. The structures and locations of all proteins in the context of the mature virion are known, with the exception of three ejection proteins. Furthermore, the changes that occur to the proteins residing in the mature virion upon DNA release are not fully understood. We used cryogenic electron microscopy to obtain what is, to our knowledge, the first asymmetric reconstruction of mature bacteriophage P22 after double-stranded DNA has been extruded from the capsid-a state representative of one step during viral infection. Results of icosahedral and asymmetric reconstructions at estimated resolutions of 7.8 and 12.5 Å resolutions, respectively, are present

SUBMITTER: McNulty R 

PROVIDER: S-EPMC5883611 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature