A New Method of Assessing Lipid Mixtures by ³¹P Magic-Angle Spinning NMR.

ABSTRACT: A variety of lipids that differ by their chains and headgroups are found in biomembranes. In addition to studying the overall membrane phase, determination of the structure, dynamics, and headgroup conformation of individual lipids in the mixture would be of great interest. We have thus developed, to our knowledge, a new approach using solid-state ³¹P NMR, magic-angle spinning, and chemical-shift anisotropy (CSA) recoupling, using an altered version of the recoupling of chemical shift anisotropy (ROCSA) pulse sequence, here penned PROCSA. The resulting two-dimensional spectra allowed the simultaneous measurement of the isotropic chemical shift and CSA of each lipid headgroup, thus providing a valuable measure of its dynamics and structure. PROCSA was applied to mixtures of phosp

SUBMITTER: Warschawski DE 

PROVIDER: S-EPMC5883613 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature