Mutations of DnaA-boxes in the oriR region increase replication frequency of the MiniR1-1 plasmid.

ABSTRACT:

Background

The MiniR1-1 plasmid is a derivative of the R1 plasmid, a low copy cloning vector.

Results

Nucleotide sequencing analysis shows that the MiniR1-1 plasmid is a 6316 bp circular double-stranded DNA molecule with an oriR1 (origin for replication). The plasmid carries the repA, tap, copA and bla genes, and genes for ORF1 and ORF2. MiniR1-1 contains eight DnaA-binding sites (DnaA-boxes). DnaA-box1 is in the oriR1 region and fully matched to the DnaA-box consensus sequence, and DnaA-box8, with one mismatch, is close to the copA gene. The presence of the MiniR1-1 plasmid leads to an accumulation of the D-period cells and an increase in cell size of slowly growing Escherichia coli cells, suggesting that the presence of MiniR1-1 delays cell division. Mutations in the MiniR

SUBMITTER: Yao Y 

PROVIDER: S-EPMC5883639 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature