Time-lapse microscopic observation of non-dividing cells in cultured human osteosarcoma MG-63 cell line.

ABSTRACT: Cancer stem cells resemble normal tissue-specific stem cells in many aspects, such as self-renewal and plasticity. Like their non-malignant counterparts, cancer stem cells are suggested to exhibit a relative quiescence. The established cancer cell lines reportedly harbor slow-proliferating cells that are positive for some cancer stem cells markers. However, the fate of these cells and their progeny remains unknown. We used time-lapse microscopy and the contrast-based segmentation algorithm to identify and monitor actively dividing and non-dividing cells in human osteosarcoma MG-63 cell line. Within the monitored field of view the non-dividing cells were represented by three cells that never divided, and one cell that attempted to divide, but failed cytokinesis, and later, after significant

SUBMITTER: Dosch J 

PROVIDER: S-EPMC5884383 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature