RABDAM: quantifying specific radiation damage in individual protein crystal structures.

ABSTRACT: Radiation damage remains one of the major limitations to accurate structure determination in protein crystallography (PX). Despite the use of cryo-cooling techniques, it is highly probable that a number of the structures deposited in the Protein Data Bank (PDB) have suffered substantial radiation damage as a result of the high flux densities of third generation synchrotron X-ray sources. Whereas the effects of global damage upon diffraction pattern reflection intensities are readily detectable, traditionally the (earlier onset) site-specific structural changes induced by radiation damage have proven difficult to identify within individual PX structures. More recently, however, development of the B_Damage metric has helped to address this problem. B_Damage

SUBMITTER: Shelley KL 

PROVIDER: S-EPMC5884390 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature