Maintenance of the Epithelial Barrier and Remodeling of Cell-Cell Junctions during Cytokinesis.

ABSTRACT: Epithelial integrity and barrier function must be maintained during the complex cell shape changes that occur during cytokinesis in vertebrate epithelial tissue. Here, we investigate how adherens junctions and bicellular and tricellular tight junctions are maintained and remodeled during cell division in the Xenopus laevis embryo. We find that epithelial barrier function is not disrupted during cytokinesis and is mediated by sustained tight junctions. Using fluorescence recovery after photobleaching (FRAP), we demonstrate that adherens junction proteins are stabilized at the cleavage furrow by increased tension. We find that Vinculin is recruited to the adherens junction at the cleavage furrow, and that inhibiting recruitment of Vinculin by expressing a dominant-negative mutant increases t

SUBMITTER: Higashi T 

PROVIDER: S-EPMC5884674 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature