Age-dependent oxidation of extracellular cysteine/cystine redox state (E_h(Cys/CySS)) in mouse lung fibroblasts is mediated by a decline in Slc7a11 expression.

ABSTRACT: Aging is associated with progressive oxidation of the extracellular environment. The redox state of human plasma, defined by the concentrations of cysteine (Cys) and cystine (CySS), becomes more oxidized as we age. Recently, we showed that fibroblasts isolated from the lungs of young and old mice retain this differential phenotype; old cells produce and maintain a more oxidizing extracellular redox potential (E_h(Cys/CySS)) than young cells. Microarray analysis identified down-regulation of Slc7a11, the light subunit of the CySS/glutamate transporter, as a potential mediator of age-related oxidation in these cells. The purpose of the present study was to investigate the mechanistic link between Slc7a11 expression and extracellular E_h(Cys/CySS). Sulforaphane treatment o

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC5884717 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature