Extracellular Cyclophilin A Augments Platelet-Dependent Thrombosis and Thromboinflammation.

ABSTRACT: Cyclophilin A (CyPA) is involved in the pathophysiology of several inflammatory and cardiovascular diseases. To our knowledge, there is no specific inhibitor targeting extracellular CyPA without affecting other extracellular cyclophilins or intracellular CyPA functions. In this study, we developed an antibody-based inhibitor of extracellular CyPA and analysed its effects in vitro and in vivo. To generate a specific antibody, mice and rats were immunized with a peptide containing the extracellular matrix metalloproteinase inducer binding site and various antibody clones were selected and purified. At first, antibodies were tested for their binding capacity to recombinant CyPA and their functional activity. The clone 8H7-mAb was chosen for further experiments. 8H7-mAb reduced t

SUBMITTER: von Ungern-Sternberg SNI 

PROVIDER: S-EPMC5885247 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature