Differential Expression Profiling of Long Noncoding RNA and mRNA during Osteoblast Differentiation in Mouse.

ABSTRACT: Long noncoding RNAs (lncRNAs) are emerging as an important controller affecting metabolic tissue development, signaling, and function. However, little is known about the function and profile of lncRNAs in osteoblastic differentiation in mice. Here, we analyzed the RNA-sequencing (RNA-Seq) datasets obtained for 18 days in two-day intervals from neonatal mouse calvarial pre-osteoblast-like cells. Over the course of osteoblast differentiation, 4058 mRNAs and 3948 lncRNAs were differentially expressed, and they were grouped into 12 clusters according to the expression pattern by fuzzy c-means clustering. Using weighted gene coexpression network analysis, we identified 9 modules related to the early differentiation stage (days 2-8) and 7 modules related to the late differentiation stage (days 1

SUBMITTER: Kim M 

PROVIDER: S-EPMC5885395 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature