Signature pathway expression of xylose utilization in the genetically engineered industrial yeast Saccharomyces cerevisiae.

ABSTRACT: Haploid laboratory strains of Saccharomyces cerevisiae are commonly used for genetic engineering to enable their xylose utilization but little is known about the industrial yeast which is often recognized as diploid and as well as haploid and tetraploid. Here we report three unique signature pathway expression patterns and gene interactions in the centre metabolic pathways that signify xylose utilization of genetically engineered industrial yeast S. cerevisiae NRRL Y-50463, a diploid yeast. Quantitative expression analysis revealed outstanding high levels of constitutive expression of YXI, a synthesized yeast codon-optimized xylose isomerase gene integrated into chromosome XV of strain Y-50463. Comparative expression analysis indicated that the YXI was necessary to initiate the xylose meta

SUBMITTER: Feng Q 

PROVIDER: S-EPMC5886582 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature