Multivariate Analysis of Two-Dimensional ¹H, ¹³C Methyl NMR Spectra of Monoclonal Antibody Therapeutics To Facilitate Assessment of Higher Order Structure.

ABSTRACT: Two-dimensional (2D) ¹H-¹³C methyl NMR provides a powerful tool to probe the higher order structure (HOS) of monoclonal antibodies (mAbs), since spectra can readily be acquired on intact mAbs at natural isotopic abundance, and small changes in chemical environment and structure give rise to observable changes in corresponding spectra, which can be interpreted at atomic resolution. This makes it possible to apply 2D NMR spectral fingerprinting approaches directly to drug products in order to systematically characterize structure and excipient effects. Systematic collections of NMR spectra are often analyzed in terms of the changes in specifically identified peak positions, as well as changes in peak height and line widths. A complementary approach is to apply principal

SUBMITTER: Arbogast LW 

PROVIDER: S-EPMC5886730 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature