Lineage-Biased Hematopoietic Stem Cells Are Regulated by Distinct Niches.

ABSTRACT: The spatial localization of hematopoietic stem cells (HSCs) in the bone marrow (BM) remains controversial, with some studies suggesting that they are maintained in homogeneously distributed niches while others have suggested the contributions of distinct niche structures. Subsets of quiescent HSCs have been reported to associate with megakaryocytes (MK) or arterioles in the BM. However, these HSC subsets have not been prospectively defined. Here, we show that platelet and myeloid-biased HSCs, marked by von Willebrand factor (vWF) expression, are highly enriched in MK niches. Depletion of MK selectively expands vWF⁺ HSCs, whereas the depletion of NG2⁺ arteriolar niche cells selectively depletes vWF^- lymphoid-biased HSCs. In addition, MK depletion compromises

SUBMITTER: Pinho S 

PROVIDER: S-EPMC5886750 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature