Rice In Vivo RNA Structurome Reveals RNA Secondary Structure Conservation and Divergence in Plants.

ABSTRACT: RNA secondary structure plays a critical role in gene regulation. Rice (Oryza sativa) is one of the most important food crops in the world. However, RNA structure in rice has scarcely been studied. Here, we have successfully generated in vivo Structure-seq libraries in rice. We found that the structural flexibility of mRNAs might associate with the dynamics of biological function. Higher N⁶-methyladenosine (m⁶A) modification tends to have less RNA structure in 3' UTR, whereas GC content does not significantly affect in vivo mRNA structure to maintain efficient biological processes such as translation. Comparative analysis of RNA structurome between rice and Arabidopsis revealed that higher GC content does not lead to stronger structure and less RNA structural flexibil

SUBMITTER: Deng H 

PROVIDER: S-EPMC5886760 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature