Mapping human pluripotent stem cell differentiation pathways using high throughput single-cell RNA-sequencing.

ABSTRACT:

Background

Human pluripotent stem cells (hPSCs) provide powerful models for studying cellular differentiations and unlimited sources of cells for regenerative medicine. However, a comprehensive single-cell level differentiation roadmap for hPSCs has not been achieved.

Results

We use high throughput single-cell RNA-sequencing (scRNA-seq), based on optimized microfluidic circuits, to profile early differentiation lineages in the human embryoid body system. We present a cellular-state landscape for hPSC early differentiation that covers multiple cellular lineages, including neural, muscle, endothelial, stromal, liver, and epithelial cells. Through pseudotime analysis, we construct the developmental trajectories of these progenitor cells and reveal the gene expression dynamics i

SUBMITTER: Han X 

PROVIDER: S-EPMC5887227 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature