Positron emission tomography of cerebral angiogenesis and TSPO expression in a mouse model of chronic hypoxia.

ABSTRACT: The present study aimed to examine whether positron emission tomography (PET) could evaluate cerebral angiogenesis. Mice were housed in a hypoxic chamber with 8-9% oxygen for 4, 7, and 14 days, and the angiogenic responses were evaluated with a radiotracer, ⁶⁴Cu-cyclam-RAFT-c(-RGDfK-)₄, which targeted α_Vβ₃ integrin and was imaged with PET. The PET imaging results showed little uptake during all of the hypoxic periods. Immunofluorescence staining of the β₃ integrin, CD61, revealed weak expression, while the microvessel density assessed by CD31 staining increased with the hypoxic duration. These observations suggest that the increased vascular density originated from other types of vascular remodeling, unlike angiogenic sprouting. We the

SUBMITTER: Kanno I 

PROVIDER: S-EPMC5888851 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature