Glutathione S-Transferase Protein Expression in Different Life Stages of Zebrafish (Danio rerio).

ABSTRACT: Zebrafish is a widely used animal model in biomedical sciences and toxicology. Although evidence for the presence of phases I and II xenobiotic defense mechanisms in zebrafish exists on the transcriptional and enzyme activity level, little is known about the protein expression of xenobiotic metabolizing enzymes. Given the important role of glutathione S-transferases (GSTs) in phase II biotransformation, we analyzed cytosolic GST proteins in zebrafish early life stages and different organs of adult male and female fish, using a targeted proteomics approach. The established multiple reaction monitoring-based assays enable the measurement of the relative abundance of specific GST isoenzymes and GST classes in zebrafish through a combination of proteotypic peptides and peptides shared within t

SUBMITTER: Tierbach A 

PROVIDER: S-EPMC5888913 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature