Multiple target autoantigens on endothelial cells identified in juvenile dermatomyositis using proteomics.

ABSTRACT: Objective:Although generally classified within the group of inflammatory myopathies, JDM displays many pathological features of vasculitis. Previous work has shown that AECA are abundant in other forms of vasculitis. We therefore investigated whether such antibodies might also be detected in JDM. Methods:We screened plasma from children with JDM for the presence of AECA by western blotting and 2D gel electrophoresis (2DE) using proteins extracted from human aortic endothelial cells as the substrate. We performed mass spectrometry to identify candidate antigens from 2DE gels and used ELISA to confirm the presence of specific antibodies. Results:We identified 22 candidate target autoantigens for AECA probed with JDM plasma. Interestingly, 17 of these 22 target antigens were proteins associat

SUBMITTER: Karasawa R 

PROVIDER: S-EPMC5888918 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature