Determination of Protein Haptenation by Chemical Sensitizers Within the Complexity of the Human Skin Proteome.

ABSTRACT: Skin sensitization associated with the development of allergic contact dermatitis occurs via a number of specific key events at the cellular level. The molecular initiating event (MIE), the first in the sequence of these events, occurs after exposure of the skin to an electrophilic chemical, causing the irreversible haptenation of proteins within skin. Characterization of this MIE is a key step in elucidating the skin sensitization adverse outcome pathway and is essential to providing parameters for mathematical models to predict the capacity of a chemical to cause sensitization. As a first step to addressing this challenge, we have exposed complex protein lysates from a keratinocyte cell line and human skin tissue with a range of well characterized sensitizers, including dinitrochlorobenz

SUBMITTER: Parkinson E 

PROVIDER: S-EPMC5889026 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature