Quantitative Proteomics Based on Optimized Data-Independent Acquisition in Plasma Analysis.

ABSTRACT: The advent of high-resolution and frequency mass spectrometry has ushered in an era of data-independent acquisition (DIA). This approach affords enormous multiplexing capacity and is particularly suitable for clinical biomarker studies. However, DIA-based quantification of clinical plasma samples is a daunting task due to the high complexity of clinical plasma samples, the diversity of peptides within the samples, and the high biologic dynamic range of plasma proteins. Here we applied DIA methodology, including a highly reproducible sample preparation and LC-MS/MS analysis, and assessed its utility for clinical plasma biomarker detection. A pancreatic cancer-relevant plasma spectral library was constructed consisting of over 14 000 confidently identified peptides derived from over 2300 pla

SUBMITTER: Nigjeh EN 

PROVIDER: S-EPMC5889294 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature