Functional Surface Display of Laccase in a Phenol-Inducible Bacterial Circuit for Bioremediation Purposes

ABSTRACT:

Background

Phenolic compounds, which are produced routinely by industrial and urban activities, possess dangers to live organisms and environment. Laccases are oxidoreductase enzymes with the ability of remediating a wide variety of phenolic compounds to more benign molecules. The purpose of the present research is surface display of a laccase enzyme with adhesin involved in diffuse adhesion (AIDA-I) autotransporter system on the surface of Escherichia coli cells for bioremediation of phenolic compounds.

Methods

The expression of laccase was regulated by a phenol-responsive promoter (a σ54 promoter). The constitutively-expressed CapR transcription activator was able to induce laccase expression in the presence of phenolic compounds.

Results

Western blot analysis showe

SUBMITTER: Fathi-Roudsari M 

PROVIDER: S-EPMC5889505 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature