An RNAi based screen in Drosophila larvae identifies fascin as a regulator of myoblast fusion and myotendinous junction structure.

ABSTRACT:

Background

A strength of Drosophila as a model system is its utility as a tool to screen for novel regulators of various functional and developmental processes. However, the utility of Drosophila as a screening tool is dependent on the speed and simplicity of the assay used.

Methods

Here, we use larval locomotion as an assay to identify novel regulators of skeletal muscle function. We combined this assay with muscle-specific depletion of 82 genes to identify genes that impact muscle function by their expression in muscle cells. The data from the screen were supported with characterization of the muscle pattern in embryos and larvae that had disrupted expression of the strongest hit from the screen.

Results

With this assay, we showed that 12/82 tested genes regulate mu

SUBMITTER: Camuglia JM 

PROVIDER: S-EPMC5889537 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature