Evaluation of the effects of ascorbic acid on metabolism of human mesenchymal stem cells.

ABSTRACT: BACKGROUND:Mesenchymal stem cells (MSCs) are multipotent cells holding much promise for applications in regenerative medicine. However, with problems such as aging, increases in heteroploid cells, genomic instability, and reduced maintenance of stemness, more stable culturing methods and the production of MSCs with an improved therapeutic effect are desired. Ascorbic acid (AsA), which is a cofactor for a variety of enzymes and has an antioxidant effect, cannot be synthesized by certain animals, including humans. Nevertheless, little attention has been paid to AsA when culturing MSCs. METHODS:We analyzed the effect of adding AsA to the culture medium on the proliferation and metabolism of human MSCs by serial analysis of gene expression and metabolome analysis. RESULTS:We found that AsA pro

SUBMITTER: Fujisawa K 

PROVIDER: S-EPMC5889584 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature