Ultrastructure of Shewanella oneidensis MR-1 nanowires revealed by electron cryotomography.

ABSTRACT: Bacterial nanowires have garnered recent interest as a proposed extracellular electron transfer (EET) pathway that links the bacterial electron transport chain to solid-phase electron acceptors away from the cell. Recent studies showed that Shewanella oneidensis MR-1 produces outer membrane (OM) and periplasmic extensions that contain EET components and hinted at their possible role as bacterial nanowires. However, their fine structure and distribution of cytochrome electron carriers under native conditions remained unclear, making it difficult to evaluate the potential electron transport (ET) mechanism along OM extensions. Here, we report high-resolution images of S. oneidensis OM extensions, using electron cryotomography (ECT). We developed a robust method for fluorescence

SUBMITTER: Subramanian P 

PROVIDER: S-EPMC5889646 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature