Ion Permeation Mechanism in Epithelial Calcium Channel TRVP6.

ABSTRACT: Calcium is the most abundant metal in the human body that plays vital roles as a cellular electrolyte as well as the smallest and most frequently used signaling molecule. Calcium uptake in epithelial tissues is mediated by tetrameric calcium-selective transient receptor potential (TRP) channels TRPV6 that are implicated in a variety of human diseases, including numerous forms of cancer. We used TRPV6 crystal structures as templates for molecular dynamics simulations to identify ion binding sites and to study the permeation mechanism of calcium and other ions through TRPV6 channels. We found that at low Ca²⁺ concentrations, a single calcium ion binds at the selectivity filter narrow constriction formed by aspartates D541 and allows Na⁺ permeation. In the presence of io

SUBMITTER: Sakipov S 

PROVIDER: S-EPMC5890290 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature