Visualizing cellulase adsorption and quantitatively determining cellulose accessibility with an updated fungal cellulose-binding module-based fluorescent probe protein.

ABSTRACT:

Background

Cellulose accessibility to cellulases (CAC) is a direct factor determining the enzymatic digestibility of lignocellulosic cellulose. Improving CAC by pretreatment is a prerequisite step for the efficient release of fermentable sugars from biomass cell wall. However, conventional methods to study the porosimetry of solid materials showed some limitations to be used for investigating CAC. In this work, an updated novel fusion protein comprising a fungal cellulose-binding module (CBM) from Cel7A cellobiohydrolase I (CBH I) of Trichoderma reesei QM6 and a di-green fluorescent protein (GFP₂) was constructed for quantitative determination of CAC.

Results

The obtained probe protein had similar molecular size (e.g., weight) with that of Cel7A and could g

SUBMITTER: Li T 

PROVIDER: S-EPMC5890345 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature