Isolation and characterization of hepatitis C virus resistant to a novel phenanthridinone derivative.

ABSTRACT:

Background

The novel phenanthridinone derivative HA-719 has recently been identified as a highly potent and selective inhibitor of hepatitis C virus replication. To elucidate its mechanism of inhibition, we have isolated and analyzed a clone of hepatitis C virus replicon cells resistant to HA-719.

Methods

To isolate HA-719-resistant replicon cells, Huh-7 cells containing subgenomic hepatitis C virus replicons (genotype 1b) with a luciferase reporter (LucNeo#2) were cultured in the presence of G418 and escalating concentrations of HA-719. After several passages, total RNA was extracted from the growing cells, and Huh-7 cells were transfected with the extracted RNA. Limiting dilution of the transfected cells was performed to obtain an HA-719-resistant clone.

Results

The

SUBMITTER: Ito W 

PROVIDER: S-EPMC5890506 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature