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Effect of sialidase fusion protein (DAS 181) on human metapneumovirus infection of Hep-2 cells.

ABSTRACT:

Methods

Hep-2 cells were preincubated with DAS181 or control DAS185 (a mutated sialidase) prior to inoculation with human metapneumovirus strains. Infectivity was assessed by a cell-based ELISA quantitating human metapneumovirus matrix protein. The effect of DAS181 on binding of recombinant G attachment protein was also determined.

Results

DAS181 blocked infection of human metapneumovirus strains A2, B1, and B2 at low concentrations. No effect of DAS185 was observed. Binding of MPV G protein to Hep-2 cells was also markedly inhibited by preincubation of cells with DAS181.

Conclusions

These results suggest that human metapneumovirus may utilize sialic acids as an entry cofactor. DAS181 may thus represent a new therapeutic agent useful for the treatment of human metapneumovirus.

SUBMITTER: Thammawat S

PROVIDER: S-EPMC5890507 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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Publications

Effect of sialidase fusion protein (DAS 181) on human metapneumovirus infection of Hep-2 cells.

Thammawat Sutthiwan S Sadlon Tania A TA Adamson Penelope P Gordon David L DL

Antiviral chemistry & chemotherapy 20151201 5-6

<h4>Methods</h4>Hep-2 cells were preincubated with DAS181 or control DAS185 (a mutated sialidase) prior to inoculation with human metapneumovirus strains. Infectivity was assessed by a cell-based ELISA quantitating human metapneumovirus matrix protein. The effect of DAS181 on binding of recombinant G attachment protein was also determined.<h4>Results</h4>DAS181 blocked infection of human metapneumovirus strains A2, B1, and B2 at low concentrations. No effect of DAS185 was observed. Binding of MP ...[more]

PMID: 27620888

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