Antibiotic susceptibility and molecular analysis of invasive Haemophilus influenzae in Canada, 2007 to 2014.

ABSTRACT:

Background

Previously we studied the antibiotic susceptibility of invasive Haemophilus influenzae collected in Canada from 1990 to 2006 and characterized isolates by serotype, MLST and ftsI gene sequencing for significant PBP3 mutations.

Objectives

To provide an update based on isolates collected from 2007 to 2014.

Methods

A total of 882 case isolates were characterized by serotype using slide agglutination and PCR. MLST was carried out to determine ST. Isolates were tested for β-lactamase production, presence of significant PBP3 mutations and antibiotic susceptibility by disc diffusion against 14 antibiotics. MIC values of three antibiotics were determined for 316 isolates using microbroth dilution.

Results

Non-typeable H. influenzae accounted for 54.6% of the

SUBMITTER: Tsang RSW 

PROVIDER: S-EPMC5890693 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature