Structural modification of LPS in colistin-resistant, KPC-producing Klebsiella pneumoniae.

ABSTRACT:

Background

Colistin resistance in Klebsiella pneumoniae typically involves inactivation or mutations of chromosomal genes mgrB, pmrAB or phoPQ, but data regarding consequent modifications of LPS are limited.

Objectives

To examine the sequences of chromosomal loci implicated in colistin resistance and the respective LPS-derived lipid A profiles using 11 pairs of colistin-susceptible and -resistant KPC-producing K. pneumoniae clinical strains.

Methods

The strains were subjected to high-throughput sequencing with Illumina HiSeq. The mgrB gene was amplified by PCR and sequenced. Lipid profiles were determined using MALDI-TOF MS.

Results

All patients were treated with colistimethate prior to the isolation of colistin-resistant strains (MIC >2 mg/L). Seven of 11 coli

SUBMITTER: Leung LM 

PROVIDER: S-EPMC5890713 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature