A comparative study of monoclonal antibodies. 1. Phase behavior and protein-protein interactions.

ABSTRACT: Protein phase behavior is involved in numerous aspects of downstream processing, either by design as in crystallization or precipitation processes, or as an undesired effect, such as aggregation. This work explores the phase behavior of eight monoclonal antibodies (mAbs) that exhibit liquid-liquid separation, aggregation, gelation, and crystallization. The phase behavior has been studied systematically as a function of a number of factors, including solution composition and pH, in order to explore the degree of variability among different antibodies. Comparisons of the locations of phase boundaries show consistent trends as a function of solution composition; however, changing the solution pH has different effects on each of the antibodies studied. Furthermore, the types of dense phases fo

SUBMITTER: Lewus RA 

PROVIDER: S-EPMC5891218 | biostudies-literature | 2015 Jan-Feb

REPOSITORIES: biostudies-literature