Optimal tracers for parallel labeling experiments and ¹³C metabolic flux analysis: A new precision and synergy scoring system.

ABSTRACT: ¹³C-Metabolic flux analysis (¹³C-MFA) is a widely used approach in metabolic engineering for quantifying intracellular metabolic fluxes. The precision of fluxes determined by ¹³C-MFA depends largely on the choice of isotopic tracers and the specific set of labeling measurements. A recent advance in the field is the use of parallel labeling experiments for improved flux precision and accuracy. However, as of today, no systemic methods exist for identifying optimal tracers for parallel labeling experiments. In this contribution, we have addressed this problem by introducing a new scoring system and evaluating thousands of different isotopic tracer schemes. Based on this extensive analysis we have identified optimal tracers for ¹³C-MFA. The best sin

SUBMITTER: Crown SB 

PROVIDER: S-EPMC5891732 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature