Molecular screening of unculturable bacteria present in men with reproductive failure.

ABSTRACT: Objective:  To determine the presence of unculturable bacteria using polymerase chain reaction (PCR) in infertile men with pyosperrmia. Design:  Perspective clinical study. Setting:  The study took place at the Department of Reproductive Medicine, Owaisi Hospital and Research Center; In vitro Fertilization Unit, Mahavir Hospital and Research Center; Center for Cellular and Molecular Biology; and Bharat Biotech Foundation, Hyderabad, India. Patients:  A total of 68 infertile men and 15 donors, all with no symptoms of genito-urinary tract infections and sterile semen cultures were included in the study. Interventions:  None. Main outcome measures:  Screening bacteria using routine bacterial cultures and PCR based screening with universal eubacterial

SUBMITTER: Anuradha P 

PROVIDER: S-EPMC5891753 | biostudies-literature | 2004 Jun

REPOSITORIES: biostudies-literature