Semi-nested polymerase chain reaction-based detection of Babesia spp. in small ruminants from Northwest of Iran.

ABSTRACT:

Aim

The present study aimed to detect Babesia ovis and Babesia motasi in the blood samples of sheep and goats from Northwest of Iran by the semi-nested polymerase chain reaction (PCR) technique.

Materials and methods

A total of 166 whole blood samples (including 123 sheep and 43 goats) were collected. In the first stage, the PCR was performed to amplify a piece of 18S rRNA gene of Babesia and Theileria genera. Then, semi-nested PCR was carried out on all PCR products to differentiate B. ovis and B. motasi.

Results

The PCR indicated that totally, 19 (11.44%) out of 166 samples were positive for Babesia or Theileria spp. The semi-nested PCR showed that 38 samples (22.89%) were positive only for B. ovis. No s

SUBMITTER: Bazmani A 

PROVIDER: S-EPMC5891838 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature